ClubLocationLeagueTierFounded
AFC Sudbury—Southern League - Premier Division Central—1999AFC Telford United—Southern League - Premier Division Central—2004Alvechurch—Southern League - Premier Division Central—1929Ascot United—Southern League - Premier Division South—1965Aylesbury United—Southern League - Division One Central—1897Banbury United—Southern League - Premier Division Central—1931Barton Rovers—Southern League - Division One Central—1898Barwell—Southern League - Premier Division Central—1992Bashley—Southern League - Division One South—1947Basingstoke Town—Southern League - Premier Division South—1896Beaconsfield Town—Southern League - Division One Central—1994Bedford Town—Southern League - Premier Division Central—1908Bemerton Heath Harlequins—Southern League - Division One South—1989Berkhamsted—Southern League - Division One Central—2009Bideford—Southern League - Division One South—1890Biggleswade Town—Southern League - Premier Division Central—1874Bishop's Cleeve—Southern League - Division One South—1905Bracknell Town—Southern League - Premier Division South—1896Bristol Manor Farm—Southern League - Division One South—1960Bromsgrove Sporting—Southern League - Premier Division Central—2009
Cashmere Technical—Southern League—2012Christchurch United—Southern League—1970Coalville Town—Southern League - Premier Division Central—1926Coastal Spirit—Southern League—2007Didcot Town—Southern League - Division One South—1907Dorchester Town—Southern League - Premier Division South—1880Dunedin City Royals—Southern League—2021Evesham United—Southern League - Division One South—1945Ferrymead Bays—Southern League—1972Frome Town—Southern League - Premier Division South—1904