ClubLocationLeagueTierFounded
AC Connecticut—USL League Two - Northeast Division—2011Ann Arbor—USL League Two - Great Lakes Division—2014Annapolis Blues—USL League Two - Mid Atlantic Division—2022Apotheos—USL League Two - Southeast Division—2021Asheville City—USL League Two - South Central Division—2016Ballard—USL League Two - Northwest Division—2021Black Rock—USL League Two - Northeast Division—2013Boston Bolts—USL League Two - Northeast Division—2015Boston City—USL League Two - Northeast Division—2015Brave SC—USL League Two - Southeast Division—2008Capital FC Atletico—USL League Two - Northwest Division—2008Cedar Stars Rush—USL League Two - Metropolitan Division—2018Charlotte Eagles—USL League Two - South Atlantic Division—1991Charlottesville Blues—USL League Two - Chesapeake Division—UNKChristos—USL League Two - Chesapeake Division—1997Dalton Red Wolves—USL League Two - South Central Division—2018Dayton Dutch Lions—USL League Two - Valley Division—2009Des Moines Menace—USL League Two - Heartland Division—1994FA Euro—USL League Two - Metropolitan Division—2013FC Buffalo—USL League Two - Valley Division—2009
FC Manitoba—USL League Two - Deep North Division—2020FC Olympia—USL League Two - Northwest Division—2014FC Tucson—USL League Two - Southwest Division—2010Flatirons—USL League Two - Mountain Division—2017Flint City Bucks—USL League Two - Great Lakes Division—1995Florida Elite SA—USL League Two - Southeast Division—2014Fort Wayne FC—USL League Two - Valley Division—2019Houston FC—USL League Two - Lone Star Division—2016Houston Sur—USL League Two - Lone Star Division—2021Kalamazoo FC—USL League Two - Great Lakes Division—2015