ClubLocationLeagueTierFounded
Alliance United—League1 Ontario—2017Burlington SC—League1 Ontario—1962FC London—League1 Ontario Championship—2008Guelph United—League1 Ontario Championship—2020Hamilton United—League1 Ontario Championship—2013Inter Toronto Pathway—League1 Ontario Championship—2024Master's FA—League1 Ontario Championship—2009North Mississauga—League1 Ontario Championship—1982North Toronto Nitros—League1 Ontario—1980Oakville SC—League1 Ontario—2024Pickering FC—League1 Ontario Championship—2014Scrosoppi—League1 Ontario—2020Sigma—League1 Ontario—2005Simcoe County Rovers—League1 Ontario—2021St. Catharines Roma Wolves—League1 Ontario—1967Unionville Milliken—League1 Ontario—1976Vaughan Azzurri—League1 Ontario—2014Whitby—League1 Ontario Championship—2015Windsor City—League1 Ontario Championship—2004Woodbridge Strikers—League1 Ontario—1976